摘要
目的：评价重庆武隆县不同立地条件青蒿样品的质量，为重庆市青蒿药材产业化发展建立一种方便、快捷的测定青蒿素含量的方法。方法：于青蒿采收期在武隆县青蒿种植基地不同立地条件下取样，样品经过烘干、粉碎、微波提取和颜色反应等步骤，通过紫外分光光度（UV—Vis）法在290nm波长处测定吸光度，根据标准溶液回归曲线计算青蒿素含量和评价青蒿品质。结果：青蒿素检测浓度在8～40mg·mL^-1范围内与吸光度呈良好线性关系（r=0．9998）；平均回收率为99.98％，RSD=1．48％（n=9）。结论：立地条件对青蒿质量有一定程度的影响；UV—Vis法简便、快速、重现性好，可用于青蒿的质量控制。
OBJECTIVE： To evaluate the quality of Arternisia annua L. samples planted in different site condition in Chongqing Wulong county and to provide a fast and convenient assaying of artemisinin in Arternisia annua L. samples in the industrial development of this Chinese herbal medicine. METHODS： The Arternisia annua L. planted in different site condition in Chongqing Wulong county were sampled during harvesting time, which were subjected to many processing steps such as drying by baking, crushing, microwave extraction and color reaction etc. Then the absorbance of the test liquid was detected by UV - spectrophotometry at a wavelength of 290 nm. Finally Artemisinin content was computed based on the regression curve of standard solution and the quality of the Arternisia annua L. samples was evaluated. RESULTS： The liner concentration range of Artemisinin was 8～40 mg · mL^-1 （r = 0.999 8） with an average recovery rate of 99.98% （RSD = 1.48%, n = 9） .CONCLUSION： The results showed that site condition did have effect on the quality of Arternisia annua L. to some degree. UV-Vis method is simple, rapid and reproducible, and suitable for the quality control of Artemisia annua L. samples.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第6期438-440,共3页
China Pharmacy
关键词
青蒿
青蒿素
紫外分光光度法
质量评价
Artemisia annua L.
Artemesinin
UV-spectrophotometry
Quality evaluation
