摘要
依据5'-鸟苷酸钠、5'-肌苷酸钠和5'-尿苷酸钠三组分的比值光谱特征,以尿苷酸钠为干扰组分时,选择258、285、244、280nm作为测定鸟苷酸钠和肌苷酸钠的波长;以肌苷酸钠为干扰组分时,选择211、241nm作为测定尿苷酸钠的波长。结果显示鸟苷酸钠在0.15～5mg/100ml,肌苷酸钠在0.2～6mg/100ml,尿苷酸钠在0.25～4mg/100ml范围具有良好的线性关系,平均回收率分别为100.2%、100.2%和99.5%。本法具有测定波长少、计算简单、光谱"分离"能力强以及能在低档分光光度计上实现、易于推广等特点。
According to the respective features of the ratio spectra of the guanosine 5＇-monophosphate（5＇-UMP）, inosine 5＇- monophosphate（5＇-IMP） and uridine 5＇-monophosphate（5＇-UMP） and taking 5＇-UMP as disturbance component, 258, 285, 244 and 280 nm were chosen as the determining wavelengths of the 5＇-GMP and 5＇-IMP. Taking the 5＇-IMP as disturbance component, 211 and 241 nm were chosen as the determining wavelengths of the 5＇-UMP. It showed that for the three components good linear correlationships exit as 5＇-GMP（0.15 -5 mg/100 ml）, 5＇-IMP（0.2-6 mg/100 ml）, 5＇-UMP （0.25-4 mg/ 100 ml）. The average recoveries are 100.2%, 100.2% and 99.5% respectively. The method is simple and practical with fewer determination wavelengths and powerful spectral resolution and can be performed on a lower level instrument. It is easy to generalize application.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第1期225-227,共3页
Food Science
关键词
双波长比值光谱法
5'-鸟苷酸钠
5'-肌苷酸钠
5'-尿苷酸钠
dual-wavelength ratio spectrometry
guanosine 5＇-monophosphate（G）
inosine 5＇-monophosphate（I）
uridine 5＇-monophosphate（U）
