摘要
目的:分析化疗后骨肉瘤的影像学变化和骨肉瘤细胞中增殖细胞核抗原(PCNA)及P-糖蛋白(P-gp)表达的相关性,探讨其临床意义。方法:对23例骨肉瘤患者于术前化疗前后行影像学检查,应用免疫组化技术检测化疗前后标本中PCNA、P-gp的表达,计算影像学评分及化疗后肿瘤细胞坏死率(TCNR)。结果:化疗后影像学评分明显比化疗前高,并且影像学评分越高,TCNR越高、预后越好。化疗后PCNA阳性表达率由87.0%降低到47.5%,P-gp阳性表达率由13.0%升高到47.8%,PCNA、P-gp阳性表达率越低预后越好,并且PCNA和P-gp的表达存在相关性。化疗后影像学评分高的患者PCNA、P-gp的阳性表达率低。结论:从细胞分子水平证实了骨肉瘤化疗后的影像学征象是以肿瘤细胞生物学行为为基础的,通过分析化疗后影像学征象和肿瘤中PCNA、P-gp的表达情况,可以预测化疗疗效,指导临床采用正确的治疗方法。
Objective：To study the relationship between radiological changes of osteesarcoma and the expression of PCNA, P-gp in tumor cells after chemotherapy.Methods： Dynamical radiological examination was done in 23 patients during prcoperative chemotherapy. Using immtmohistochcmical staining, the expressions of PCNA,P-gp were examined. Radiological scores and TCNR were calculated.Resalts： The post-chemotherapy radiological scores were much higher than those of pre-chcmothcrapy. The positive expression rate of PCNA after cbemothcrapy was decreased and the rate of P-gp was increased. The higher of the positive expression rate of PCNA and P-gp, the better of the prognosis, and there was relationship between the expression of PCNA and P-gp. The negative relationship existed between the radiological scores and expressions of PCNA and P-gp. Conclusion： At the cell molecular level, it was confirmed that the radiolngical signs of osteosareoma after chemotherapy were on the base of tumor cell biological behaviour. We could predict the therapeutic effect by analyzing the radiological signs and the expression of PCNA,P-gp.
出处
《医学影像学杂志》
2008年第1期79-83,共5页
Journal of Medical Imaging
关键词
骨肉瘤
化疗
影像学
免疫组织化学
Osteosareoma
Chemotherapy
Radiography
Immunohistochemistry
PCNA
P-gp
