摘要
目的建立适用于影像研究的人类结肠癌肝转移瘤裸鼠模型,观察常规MRI表现及扩散加权成像(DWI)图像质量。方法将对数生长期的人类结肠癌SW480制成细胞悬液(1×107/ml),取0.5ml注射至2只裸鼠胁部皮下制作种瘤鼠。将种瘤鼠麻醉,切除肿瘤制成大小约1mm3组织块,将小肿瘤块种植于36只(雌雄各半)麻醉状态下的裸鼠肝脏制成结肠癌肝转移瘤荷瘤裸鼠模型。给予荷瘤鼠行MRT1WI、T2WI、DWI扫描。结果2～6周后,裸鼠肝脏肿瘤长至0.7～2cm,2只伴有全肝播撒,36只裸鼠移植性人类结肠癌肝转移瘤模型成瘤率100%(36/36)。T1WI、T2WI图像均能清晰显示肝脏及肿瘤,肿瘤T1WI为等信号,T2WI为高信号。与常规T2WI图像比较,DWI图像明显变形4只,表面变形不影响肿瘤观察14只,表面无变形18只。结论本研究所建立的人类结肠癌肝转移荷瘤裸鼠模型成瘤率高,移植瘤生长良好,便于MR的常规成像与DWI观察,MRI图像可反映移植瘤的病理改变,是一种适用于分子影像学研究的动物模型。
Objective To establish a nude mouse model mimicking hepatic metastasis of human colon carcinoma and study its magnetic resonance imaging （MRI） features and the quality of diffusion-weighted images （DWI）. Methods Human colon carcinoma SW480 cells were inoculated subcutaneously （（1 ×10^7/ml, 0.5 ml） in the costal regions of 2 nude mice, and after successful tumor formation, the generated tumors were harvested and cut into tissue blocks of 1 mm3. The tissue blocks were subsequently implanted into the liver of 36 nude mice, fi＇om which TSE-T 1WI, T2WI and EPI-DWI were obtained after tumor growth. Results The success rate ofintrahepatic tumor implantation was 100% （36/36）, and the tumors grown in the liver measured 0.7 to 2 cm in diameter 2 to 6 weeks after implantation. All the tumors were clearly visualized on T1WI and T2WI images, with isointensity on T1WI and hyperintensity on T2WI. Compared with the T2WI, DWI showed conspicuous geometry deformation in 4 mice to cause difficult detection, moderate to slight geometric deformation in 14 mice which remained detectable, and no geometric deformation in 18 mice. Conclusion The nude mouse model of hepatic metastasis of SW480 colon cancer cell line, with ideal implantation and tumor growth rate, allows convenient tumor observation on MR T1WI, T2WI and DWI, and the MRI findings of the tumor are well consistent with those by pathological examination, suggesting the validity of this model for molecular imaging research of human colon cancer.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第1期97-100,共4页
Journal of Southern Medical University
基金
广州市医药卫生科技计划项目(2006-YB-033)~~
关键词
裸鼠
新生物
结肠
磁共振成像
nude mice
neoplasm
colon
magnetic resonance imaging
