摘要
目的观察胞嘧啶脱氨酶／5-氟胞嘧啶（CD／5-FC）系统热化疗对裸鼠结肠癌肝转移的作用。方法45只裸鼠随机分为3组，分别给予不同的治疗方法，观察各组肝转移率、肝转移瘤数目、病理学变化、肿瘤细胞凋亡指数，检测肿瘤组织中CD基因的表达情况。结果对照组、5-FC治疗组、5-FC热疗组平均肝脏转移瘤数和肝脏转移率分别为4．6000±1．2649、2.2000±0．9189、0．5000±0．8498；100％、40％、20％；肿瘤细胞凋亡指数平均为4．59％、9．87％和17．40％，5-FC热疗组可见较多的凋亡小体形成。结论CD／5-FC系统热化疗对裸鼠转CD基因LoVo细胞结肠癌肝转移有明显的抑制作用。
Objective To explore the effect of CD/5-FC system combined with thermotherapy on liver metastasis of colon carcinoma. Methods Forty-five nude mice were divided into 3 groups randomly. Different treatments were administrated, then liver metastasis rate, the number of liver metastasis nodules, the pathologic changes of the tumor tissues, and apoptosis index of tumor cells were analyzed in every group respectively. The expression of CD gene in tumor tissues were detected by real-time RT-PCR and Western blot. Results Liver metastasis rate, number of metastasis nodules and tumor cell apoptosis index were 100% , （4.6± 1.3 ） , 4.59% in control group, 40%, （ 2.2 ± 0.9） , 9.87% in 5-FC group and 20%, （ 0.5 ± 0.8 ） , 17.40% in 5-FC thermotherapy group respectively. Conclusion Inhibition of liver metastasis of colon cancer cells LoVo transfeeted with CD gene combined with thermotherapy can be observed evidently in nude mice.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第11期1450-1451,共2页
Chinese Journal of Experimental Surgery
基金
福建省自然科学基金资助项目（C0410045）
关键词
结肠癌
肝转移
基因治疗
Colon carcinoma
Liver metastasis
Gene theropy
