摘要
目的建立用HPLC测定仙桂胶囊中盐酸麻黄碱含量的方法。方法色谱柱：Kromasil C18（4．6mm×200mm，5μm）；流动相：甲醇-0．1％磷酸溶液（8：92）（每1000mL加三乙胺1 mL）；检测波长：210nm；流速：1．0mL·min^-1；柱温：室温。结果盐酸麻黄碱在0．02～0．20μg范围内线性关系良好，回归方程A=3854194．0410C-1882．0548，r=0．9996。平均回收率为97．44％（n=5），RSD为1．04％。结论本方法专属性强，结果可靠，重现性好，可有效控制仙桂胶囊的质量。
Objective A HPLC method was established for the determination of ephedrine hydrochloride in Xiangui capsules. Methods Kromasil C 18 column（4.6 mm× 200 mm,5 μm） was used and the mobile phase consisted of methonal-0.1% phosphoric（8：92 ）. The detection wave length was at 210nm and the flow rate was 1.0 mL·min^-1. Results The calibration curve was in good linearity over the range of 0.02 - 0.20 μg, A = 3 854 194. 041 0C - 1 882. 054 8, r = 0. 999 6. The average recovery was 97.44% and RSD was 1.04%. Conclusion The method was simple, sensitive , accurate, and could be used for the quality of Xiangui capluses.
出处
《现代中药研究与实践》
CAS
2007年第6期48-49,共2页
Research and Practice on Chinese Medicines
关键词
仙桂胶囊
盐酸麻黄碱
HPLC
Xiangui capsules
ephedrine hydrochloride
HPLC
