摘要
目的:建立双青咽喉片中绿原酸和甘草酸含量测定方法。方法:采用高效液相色谱法。色谱柱:Dikma Kromasil C_(18)柱(250 mm×4.6 mm,5 μm);绿原酸的测定,流动相为甲醇-0.08 mol·L^(-1)磷酸溶液(22:78),流速:1.0 mL·min^(-1),检测波长:326 nm;甘草酸的测定,流动相为甲醇-0.2 mol·L^(-1)酸铵-冰醋酸(66:34:1);流速:0.8 mL·min^(-1);检测波长:252 nm。结果:绿原酸在0.08～0.8μg范围内具有良好的线性关系(r=0.9999),平均加样回收率为98.90%(n=12);甘草酸的线性范围0.4～4μg(r=0.9999),平均加样回收率为99.04%(n=12);。结论:该方法简便、快速、准确,具有良好的重复性和回收率,可作为该制剂的定量分析方法。
Objective：To establish an HPLC method for the determination of chlorogenic acid （CHA） and glycyrrhizic acid（GA） in Shuangqingyanhou tablets. Methods：The separation was carried out on a Dikma Kromasil C18 column （250 mm × 4. 6 mm, 5 μm）. The determination of CHA with methanol - 0. 08 mol . L ^- 1 phosphoric acid solution（22： 78）as the mobile phase,the flow rate was 1.0 mL . min^-1 and the detection wavelength was 326 rim; The determination of GA with methanol-0. 2 mol . L^-1 ammonium acetate -glacial acetic acid（66.34：1 ）as the mobile phase,the flow rate was 0. 8 mL . min^-1 and the detection wavelength was 252 nm. Results：The calibration curves showed good linearities over the range of 0. 08 -0. 8 μg（r =0. 9999） for CHA and 0. 4 -4 μg（r =0. 9999） for GA. The average recoveries were 98.90% with for CHA and 99.04% for GA. Conclusion：This method is simple, rapid, accurate and with good repeatability and recovery, it can be used as a quantitative analysis method for this preparation.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第12期1903-1905,共3页
Chinese Journal of Pharmaceutical Analysis
基金
泸州重点科技项目(泸市科[2005]25号)
关键词
HPLC
双青咽喉片
绿原酸
甘草酸
含量测定
HPLC
Shuangqingyanhou tablets
chlorogenic acid
glycyrrhizic acid
content determination
