摘要
以高粱种子为材料,研究了不同提取剂、提取剂用量、浓度和还原剂浓度对高粱种子醇溶蛋白提取的影响.蛋白浓度测定和SDS-PAGE电泳表明,DDT浓度为4%的60%叔丁醇溶液,样品质量比提取剂用量为1∶8(g/mL)是提取高粱种子醇溶蛋白的最佳条件.该条件下,提取高粱属不同种的种子醇溶蛋白,得到的高粱属种子醇溶蛋白的"指纹"图谱,可以用来区分高粱属内的种间差异.
Effects of different reagents, dosages, concentrations, and reduction reagent concentrations on kafirin extraction from Sorghum bicolor were investigated. Measurements of protein concentration and the sodium dodecyl sulphate-polyacrylamide gel electrophoresis （SDS-PAGE） showed that 60% r-butanol plus 4% DTT at a sample-weight to extraction-solution ratio of 1：8 （g/mL） was optimum for kafirin extraction. Other sorghum species＇ alcohol-solute protein （prolamin） were extracted under this condition. It is concluded that, by studying the protein ＂fingerprint＂, different sorghum species can be discriminated from each other.
出处
《上海大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第6期746-750,共5页
Journal of Shanghai University:Natural Science Edition
基金
上海科委基金资助项目(04DZ05003)
关键词
高粱
醇溶蛋白
提取
sorghum
kafirin
extraction
