摘要
以马蔺的根茎不定芽为材料，成功地进行了马蔺根茎离体不定芽的生长、生长不定芽的分化、试管苗的生根、移栽、定植的研究，建立起马蔺的无性系。结果证明：1／2MS＋GA0．5mg／L＋BA0．3mg／L＋IAA0．5mg／L是诱导根茎不定芽生长的理想培养基；Ms＋GA0．2mg／L＋BA0．6mg／L＋IAA0．3mg／L是生长不定芽分化培养的理想培养基；1／3MS＋IAA0．4-0．6mg／L是生根培养的理想培养基；炉灰渣是试管苗移栽的理想基质。
Using the Iris lactea var. chinensis Koidz rhizomes as explant, tissue culture and clones of Iris lactea var. chinensis Koidz were established in order to study the different conditions of callus induction, bud differentiation, rooting, and transplantation of seedlings. The results showed that the medium 1/2MS ＋ GA 0.5 mg/ L ＋ BA 0.3 mg/L ＋ IAA 0.5 mg/L was the best for inducing callus from rhizomes, medium MS ＋ GA 0.2 mg/ L ＋ BA 0.6 mg/L ＋ IAA 0.3 mg/L was the most suitable for bud differentiation, medium 1/3MS ＋ IAA 0.4 0.6 mg/L was the optimum for rooting. The ideal transplantation matrix for tube plantlets was the furnace ashes.
出处
《辽宁农业科学》
2007年第6期4-6,共3页
Liaoning Agricultural Sciences
关键词
马蔺
组织培养
无性系
Iris lactea var. chinensis Koidz
Culture of tissue
Clone
