摘要
在仿自然气候条件下,采用培养苗形态指标综合分析的方法,对3种鸢尾组织培养直接成苗的培养基进行筛选。结果表明:最适合黄娃娃和不朽白一次性成苗的培养基为MS+KT 0.5 mg.L-1+IBA 1 mg.L-1+-αNAA 0.6 mg.L-1+蔗糖30 g.L-1+琼脂粉8 g.L-1+活性碳0.3%,pH 6.7;最适合血石一次性成苗的培养基为MS+KT 0.5 mg.L-1+IBA 1mg.L-1+-αNAA 0.2 mg.L-1+蔗糖30 g.L-1+琼脂粉8 g.L-1+活性碳0.3%,pH 6.7。
Under the culture condition for imitating nature climate, through the integrated analytic ways of culture seeding form target, Culture medium for culturing 3 varieties of Iris germanica into seedling by tissue directly was filtrated. The results showed ： the best adaption culture medium to I. germanica ＇ Sundoll＇ and I. germanica ＇ Immortality＇ plant in one step was MS ＋ KT 0.5 mg·L^-1 ＋ IBA 1 mg·L^-1 ＋ a-NAA 0.6 mg·L^-1 ＋ sucrose 30 g·L^-1 ＋ agar 8 g·L^-1 ＋ activated carbon 0.3 %, pH 6.7 ; the best adaption culture medium to I. germanica ＇ Bloodstone＇ plant in one step： MS ＋ KT 0.5 mg·L^-1 ＋ IBA 1 mg·L^-1 ＋ α- NAA 0.2 mg·L^-1 ＋ sucrose 30 g·L^-1 ＋ agar 8 g·L^-1 ＋ activated carbon 0.3 %, pH 6.7.
出处
《福建林业科技》
2009年第3期85-87,共3页
Journal of Fujian Forestry Science and Technology
基金
河南省科技公关项目(072102110010)
关键词
鸢尾
组织培养
直接成苗
培养基
Iris germanica.
tissue culture
culturing into seedling directly
culture medium
