摘要
目的探讨谷氨酰胺(GLN)二肽胃肠外营养对急性放射性肠炎大鼠小肠上皮细胞产生营养作用的机制。方法19只SD大鼠行全腹部放射后,随机分为三组,常规胃肠外营养组(STD组,n=6):大鼠输入常规静脉营养液;谷氨酰胺组(GLN组,n=7):大鼠输入含谷氨酰胺的静脉营养液;正常组(n=6)为未经腹部照射的正常大鼠。连续7d胃肠外营养,在实验结束前经颈静脉注入5-溴脱氧尿苷(BrdU),采用ABC方法及光镜观察小肠隐窝细胞有丝分裂相、细胞增殖核抗原(PCNA)和5-溴脱氧尿苷(BrdU)阳性细胞。结果GLN组小肠隐窝细胞有丝分裂相细胞数多于STD组(P<0.05)。GLN组小肠增殖细胞核抗原(PCNA)阳性细胞数多于STD组(P<0.05)。GLN组小肠BrdU阳性细胞数多于STD组(P<0.05)。结论GLN二肽可促使急性放射肠炎大鼠小肠上皮细胞的分裂增殖。
Objective To study the nutrient mechanism of glutamine-enriehed parenteral nutrition on rats＇ small bowel of acute radiation enterltis.Methods Nineteen SD rats were used in this study. Whole abdominal radiation was performed in the SD rats.The animals were randomly divided into three groups. Standard total parenteral antrition（STD group, n=6） and glutamine-enriehed total parenteral nutrition（GLN group,n=7）was used from intravenous infusion continuously for 7days, and normal nonirradiatod animals （n=6）. The samples of small bowel were taken after intravenous injection 5-bromodexyridine （BrdU） from internal vena for lhour.ABC immunohistoehemistry and light microscopy were used to observe intestinal crypt cell mitosis,prollferating cell nuclear antigen and bromodexyridine labelled cells.Results [ntestina] cell mitosis per erypt,proliferating cell nuclear antigen（PCNA） and bromodexyridine（BrdU） labelled cells were predominantly in crypt epithelium of small bowel in each rat,Compared with that of STD group,the numbers of cell mitosis,PCNA and Brdu labelled cell per crypt of GLN group increased significantly. Conclusion Glutamine-enriched parenteral nutrition can improve the intestinal epithelium proliferation after abdominal radiation.
出处
《实用医药杂志》
2007年第12期1493-1495,共3页
Practical Journal of Medicine & Pharmacy
