摘要
目的：观察大鼠急性肺损伤局部与主要器官脂质过氧化改变。方法：用静脉注射油酸的方法复制大鼠急性肺损伤模型。实验分对照组和油酸组，观察肺大体变化；取支气管——肺泡灌洗液（ＢＡＬＦ）、血浆、测定蛋白质及丙二醛（ＭＤＡ）含量；取心、肺、肝、肾组织测ＭＤＡ及ＳＯＤ。结果：与对照组相比：（１）肺组织严重受损；（２）支气管—肺胞灌洗液中蛋白质、ＭＤＡ含量明显增高；（３）各组织ＭＤＡ水平明显增高，ＳＯＤ活力明显减低。结论：急性肺损伤时，肺脏出现脂质过氧化损害，同时主要器官亦出现类似改变。这种损害可能是ＡＲＤＳ时造成多器官衰竭的一条重要途径。
Objective:To observe the changes of the lipid peroxidation of the ung and other major organs during the acute lung injury in rats.Methods:17 rats,weighing 200-250g,were used in this experiment,dividing into the control group(n=7)and the oleic-acid group(n=10).Acute lung injury was produced by intravenous injection of oleic-acid at the dosage of 0.1ml/kg.Pulmonary changes were observed.MDA,SOD and protein in the samples taken from the lung.BALF,heart,liver,kidney and plasma were measured respectively.Results:The oleiv-acid group showed:(1)pulmonary tissue was injured.(2)Protein and MDA content from BALF were obviously increased;(3)MDA content in the lung,heart,liver and kidney was increased and SOD activity decreased as compared with those of control group.Conclusion:Lipid peroxidaton in the lung during acute lung injury induced by oleic-acid was increased,the similar changes might appear in the heart,Liver and kidney.The mechanism causing such injury is that local toxic produts reach to the organs through the blood flow or activated PMN plug into organs microvasculature after injection of olercacid.This mechanism may also be a pathway of multiple organ failure during acute respiratory distress syndrome
出处
《长治医学院学报》
1997年第1期3-5,共3页
Journal of Changzhi Medical College
关键词
肺损伤
油酸
丙二醛
超氧化物歧化酶
Acute lung injury
Oleic-acid
Malondialdehyde
Superoxide dismutase
Multiple organ failure
