摘要
目的:观察油酸性肺损伤大鼠肝组织TNF-α、IL-6的表达,探讨油酸致肺损伤发生中细胞因子与肝损伤的相关性。方法:16只健康Wistar雄性大鼠随机分为对照组和油酸6 h组(OA6 h),2组大鼠测肺湿/干重(W/D)及血清丙氨酸氨基转移酶(ALT)、天门冬酸氨基转移酶(AST)和总胆红素(TB)的改变,应用放射免疫法测定血清及肝组织中TNF-α、IL-6蛋白含量,应用逆转录-聚合酶链反应法(RT-PCR)检测肝组织TNF-α、IL-6 mRNA的表达,同时观察肝组织形态学的变化。结果:OA6 h组肺W/D、ALT、AST和TB均高于对照组(P<0.01),OA6 h组血清和肝组织TNF-α、IL-6蛋白含量以及肝组织TNF-α、IL-6 mRNA的表达均较对照组增多(P<0.01)。结论:油酸致大鼠ARDS过程中可发生肝功能障碍,同时肝组织TNF-α、IL-6在基因和蛋白水平的表达均增强。ARDS过程中出现的肝功能障碍可能与TNF-α、IL-6的参与有关。
Objective： To observe the expression of TNF-α and IL-6 in the liver on oleic acid induced ALI rats. To discuss the relevance of cytokine and the liver injury during ARDS by oleic acid. Methods： Sixteen healthy male Wistar rats were randomly divided into control group and OA6h group. The W/D of lung was measured, Liver function （ALT, AST, TB） of each group was detected. The content of TNF-α, IL-6 protein in hepatic tissue of each group was detected by radioimmunoassay. The expressions of TNF-α, IL- 6mRNA in hepatic tissue of each group were detected by RT-PCR. The structure of liver was observed by light microscope. Results： The levels of W/D, ALT, AST, TB in the OA6h were higher than the control group （P 〈0.01）. The contents of TNF-α and IL-6 protein in blood plasma and hepatic tissue, the expression of TNF-α and IL-6mRNA in hepatic tissue increased compared with control group （P 〈0.01）. Conclusions： Functional disturbance of liver may take place during ARDS by oleic acid, the expressions of TNF-α and IL-6 increased in gene and protein level, TNF-α and IL-6 may play a role in the function disturbance of the liver during ARDS.
出处
《新疆医科大学学报》
CAS
2008年第11期1525-1527,共3页
Journal of Xinjiang Medical University
