摘要
目的探讨不同转移潜能卵巢癌细胞株蛋白表达谱差异。方法采用基于双向电泳(2-DE)-基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)蛋白质组学分析方法,对比分析具有相同来源、不同转移能力的卵巢癌细咆系 HO8910和 HO8910pm 的总蛋白表达谱差异。结果 2-DE分析发现两株细胞间有39个蛋白表达水平差异在2倍以上,MALDI-TOF-MS 鉴定出其中18种蛋白,它们涉及细胞凋亡、细胞外基质、细胞骨架、生长因子、糖酵解、蛋白质代谢和免疫系统等领域。结论肿瘤转移涉及体内多种领域多种蛋白,这些领域的蛋白相互协调作用最终共同控制细胞的转移能力。
Objective To investigate the differenal protein expression profiles of ovarian tumor cell lines with distinct metastatic abilities. Methods The ovarian cancer cell line HO8910 and HO8910pm, derived from same parental cells but exhibited different metastatic ability, were investigated by two- dimensional gel electrophoresis （2-DE）-MALDI-TOF-MS proteomic approach. Results Thirty-nine proteins were detected by 2-DE to have expression disparity levels over 2 folds between two cell lines. Eighteen of them were identified by MALDI-TOF-MS. The proteins are involved in apoptosis, extra cellular matrix （ECM）, cytoskeleton, growth factor, glycolysis, protein metabolism and immune system. Conclusion The data are valuable for the identification of differentially expressed proteins involved in the biological behavior of human ovarian cancer.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2007年第12期814-818,共5页
Chinese Journal of Pathology
