摘要
目的检测急性白血病(AL)患儿血清白细胞介素-15(IL-15)水平,了解IL-15水平与儿童AL的关系。方法确诊未治的急性淋巴细胞白血病(ALL)、急性非淋巴细胞白血病(ANLL)和非白血病组儿童各20例,分别收集血清及骨髓单个核细胞(BMMC);另收集20例正常儿童血清作对照。使用酶联免疫吸附法(ELISA)测定不同组别血清IL-15水平。结果ALL组、ANLL组、非白血病组及正常对照组血清IL-15水平分别为(34.37±2.8)ng/L,(29.61±3.2)ng/L,(117.54±3.9)ng/L,(122.23±4.2)ng/L;其中ALL组、ANLL组分别与正常对照组进行比较均有显著意义(q=3.95,4.03Pa<0.05)。ALL组、ANLL组分别与非白血病组比较均有显著意义(q=4.17,3.76Pa<0.05)。ALL组与ANLL组比较、正常对照与非白血病组比较无明显差异(Pa>0.05)。结论检测血清IL-15水平对判断白血病患儿抗肿瘤免疫状态有一定的临床意义。
Objective To investigate the levels of interleukin-15（IL-15） in children with acute leukemia and contribute to understand their function on acute leukemia about the process.Methods Every group of acute lymphocytic leukemia（ALL）,acute nonlymphocytic leukemia（ANLL） or non-leukemia group had 20 children who did not receive any treatment. Peripheral blood of every group and 20 other healthy children were aspirated .The levels of IL-15 in serum was analyzed by enzyme linked immunosorbent assay （ELISA）.Results The levels of IL-15 in ALL group,ANLL group, non-leukemia group and healthy children were （34.37±2.8） ng/L, （29.61±3.2 ）ng/L, （117.54±3.9） ng/L, （122.23±4.2） ng/L. Compared with the levels of the healthy group or the non-leukemia group, the difference of ALL or ANLL was signicant in statistics（q=3.95,4.03 Pa〈0.05）.IL-15 levels of ALL group compared with ANLL group,healthy children compared with non-leukemia group（Pa〉0.05）.Conclusion Detection of serum IL-15 levels may have clinical significance in judging the anti-tumor immune condition in children with leukemia.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第3期186-187,共2页
Journal of Applied Clinical Pediatrics
基金
广东省自然科学基金项目资助(5011591)
