摘要
目的动态检测肺炎支原体肺炎(MPP)患儿诱导痰液IL-4和INF-γ水平,分析Th1/Th2细胞因子免疫应答在MPP发生发展中的作用.评价诱导痰液方法在MPP疾病研究中的临床价值。方法MPP患儿38例.用3%的高渗盐水超声雾化吸入.诱导痰液。采用酶联免疫方法检测IL-4及IFN-γ。结果MPP患儿诱导痰IL-4含量急性期明显高于恢复期(P<0.01),重症组明显高于轻症组(P<0.01);IFN-γ含量急性期与恢复期、重症组与轻症组比较无显著差异(P>0.05)。IL-4/IFN-γ急性期明显高于恢复期,重症组明显高于轻症组(P<0.05)。恢复期IFN-γ、IL-4、IL-4/IFN-γ重症组和轻症组比较无显著差异(P>0.05)。结论IL-4及IFN-γ参与MPP发病.MPP存在Th1/Th2失衡,以Th2反应占优势。诱导痰液分析有可能成为实用、方便且较灵敏的判断MPP轻重程度的较好方法。
Objective To detect levels of IL-4 and INF-γ in induced sputum dynamically in children with mycoplasma pneamoniae pneumonia（MPP）, and to analyze the function of Th1/Th2 cell factor inmmune response in the genesis and development of MPP, so as to evaluate the clinical value of induced sputum method in MPP research. Methods There were 38 cases who were diagnoses as MPP using 3% high osmotic pressure of saline water to ultrasonic atomizing inhalation for inducing sputum. ELISA was used to detect IL- 4 and INF- γ. Results The content of 1L-4 in acute stage was higher than that in convalescence stage in induced sputum of MPP children. Severe stage was higher than mild stage, However, the comparison between acute and convalescence stage didn＇t have statistics difference in the content of INF-γ, neither did the comparison between severe and mild stage. 1L-4/INF-γ in acute stage was higher than that in cconvalescence stage.Severe stage was higher than mild stage, In comvalescence stage, the comparison of INF-γ, 1L- 4,1L- 4/INF-γ between the severe and the mild didn＇t have statistic significance. Conclusions IL-4 and INF-γ have participated in the monogcnesis of MPP. The disequilibria of Th1/Th2 is existed in MPP and Th2 reaction predominates.so indueed sputum analysis can be a better way to judge the light or heavy press degree of MPP practically, conveniently and sensitively.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2005年第10期965-967,共3页
Journal of Applied Clinical Pediatrics
