摘要
目的:建立溃结康微丸中芍药苷和黄芩苷的 HPLC 含量测定方法。方法:采用 Kromasil C_(18)柱(250 mm×4.6 mm,5.0μm),0.2%磷酸溶液(A)和乙腈(B)为流动相梯度洗脱,流速:1.0 mL·min^(-1),检测波长:230 nm,柱温为25℃。结果:芍药苷在1.749～17.49μg·mL^(-1)(r=0.9996)、黄芩苷在13.33～133.3μg·mL^(-1)(r=0.9998)范围内呈良好的线性关系,平均回收率(n=6)分别为98.2%和97.4%,RSD 分别为1.5%和1.9%。结论:本法简便、快速、准确,可用于溃结康微丸中芍药苷和黄芩苷的同时测定。
Objective ： To establish a method for the determination of paeoniflorin and baicalin in Kujiekang pepllets. Methods：The HPLC method was conducted on a Kromasil C18 column（250 mm ×4. 6 mm,5.0 μm）. The mobile phase consisted of 0. 2% phosphoric acid solution （A） and acetonitrile （ B ） with gradient elution program. The flow rate was 1 mL · min^-1 and the column temperature was 25 ℃. The detection wavelength was 230 nm. Results：The average recovery was 98.2%, RSD = 1.5% （n = 6）for paeoniflorin, the linear range was 1. 749 - 17.49 μg · mL^-1 （r =0. 9996） ,and the average recovery was 97.4% ,RSD = 1.9% （n =6） for baicalin,the linera range was 13.33 - 133.3 μg ·mL^-1 （ r = 0. 9998 ）. Conclusion： The method is simple, accurate and suitable for the determination of paeoniflorin and baicalin in Kuijiekang pepllets
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第11期1746-1748,共3页
Chinese Journal of Pharmaceutical Analysis
