摘要
目的:从Hela细胞中克隆凋亡抑制因子Survivin的编码序列,进行测序,并由此预测其HLA-A*0201限制性CTL表位。方法:设计人Survivin基因序列特异引物,通过RT-PCR扩增Survivin编码序列,构建至pGEM-T载体后,测序分析,并对其进行了HLA-A*0201限制性CTL表位抗原表位相关预测。结果:从Hela细胞中克隆得到的Survivin和Survivin-ΔEx3两种剪接体,其中Survivin-ΔEx3发生1个同义突变和三个错义突变,预测其抗原表位抗原性发生了变化。结论:寻找抗原性强、结合力高的表位,对于今后抗肿瘤多肽疫苗的研究具有重要的意义。
Objective： Survivin belongs to the apoptotic inhibitor family. The coding sequence of this gene was cloned from Hela cells and sequence,whereby to forecast its HLA-A ＊ 0201 restricted CTL epitope. Methods：With specific primers, the full-length cDNA sequences of human survivin gene were amplified by RT-PCR, and then cloned into the pGEM-T vector for sequence analysis. The result sequence was aligned with reference sequences in GeneBank by DNAMAN tools, and their HLA-A ＊ 0201 restricted CTL epitopes were predicted subsequently. Results：Two kinds of variants of survivin gene were obtained, one was survivin, and the other was survivin-ΔEx3. For special attention, the survivin-ΔEx3 variant had one synonymous mutation and three nonsynoymous mutations. The nonsynonymous mutations resulted in the changed epitope prediction results. Conclusion：The identification of the epitope with strong- antigenicity and high-affinity is significant for future research on anti-tumor peptide vaccine.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2007年第11期1044-1047,1051,共5页
Chinese Journal of Immunology
基金
国家自然科学基金(30572124)
广东省自然科学基金(5002855)
广东药学院博士启动基金(43555014)
