摘要
目的研究阿魏酸钠对缺氧脑血管内皮细胞核转录因子κB(nuclearfactor-κB,NF-κB)及IκBα表达的影响。方法建立大鼠脑皮质微血管内皮细胞(brainmicrovascularendothelialcell,BMEC)体外培养模型,并施加12h缺氧条件,分为对照组、缺氧组、阿魏酸钠组。应用MTT法测定细胞活性,采用放射免疫方法测定内皮素(endothelin-1,ET-1)含量,采用免疫细胞化学法检测BMEC的NF-κB及IκBα表达。结果阿魏酸钠(100μg/ml)能抑制缺氧诱导的ET-1释放(P<0.01)。缺氧刺激血管内皮细胞NF-κB的表达,降低IκBα的表达,阿魏酸钠可以显著抑制缺氧内皮细胞中NF-κB的表达及核移位现象,增加IκBα的表达和BMEC活性。结论阿魏酸钠对大鼠脑皮质BMEC缺氧损伤具有保护作用,机制与其减少BMEC分泌ET-1,抑制NF-κB蛋白表达,促进IκBα蛋白表达有关。
Objective To study the effect of sodium ferulate （SF） on the expressions of nuclear factor-κB （NF-κB） and inhibitory κBα（IκBα） in rat brain microvascular endothelial cells （BMEC） injured by hypoxia. Methods Brain microvascular endothelial cells from Wistar rat cerebral cortex were isolated and cultured, and then cultivated in 95% N2 and 5% CO2 for 12 h to induce hypoxia injury. The cells were divided into normal group, hypoxia group, and SF group. BMEC activity was assayed by MTF assay. Endothelin-1 （ ET-1 ） concen-tration was evaluated by radioimmunoassay. The expression of NF-κB and IκBα of BMEC were assessed by im- munocytochemistry. Results The content of ET-1 in BMEC media was increased after hypoxia, while sodium ferulate （100μg/ml） significantly decreased the content compared with hypoxia group. Immunocytochemistry indicated IκBα expression were significantly decreased in hypoxia BMEC in consistent with NF-κB expression. Conclusion Sodium ferulate may significantly reduce NF-κB of hypoxia BMEC and nuclear translocation, and increase IκBα expression and BMEC activity. It is a potent inhibitor of NF-κB in endothelial cells, which might explain its beneficial effect on injured BMEC by hypoxia.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第3期228-230,共3页
Journal of Third Military Medical University
基金
重庆市卫生局医学科研项目(2004-B-86)~~
关键词
阿魏酸钠
脑血管内皮细胞
缺氧
NF-ΚB
IΚBΑ
sodium ferulate
brain microvascular endothelial cells
hypoxia
nuclear factor-κB
IκBα
