摘要
为了探讨人巨细胞病毒（Ｈｕｍａｎｃｙｔｏｍｅｇａｌｏｖｉｒｕｓ，ＨＣＭＶ）与宫颈癌发生之间的关系，我们利用紫外线灭活的ＨＣＭＶ诱发小鼠宫颈癌的动物模型进行了研究。经紫外线灭活的ＨＣＭＶ液接种于小鼠宫颈部，３次／周×８周，接着以巴豆油按同样的方法２次／周×４周进行处理。同时设立ＨＣＭＶ及人胚肺细胞ＨＥＬ培养液对照组等。经２０个月后，折颈处死动物，取出完整的生殖道作病理学检测，结果发现：ＨＣＭＶ组宫颈癌前病变率为２７．８％（２３／８３），癌发率为２０．５％（１７／８３）；ＨＥＬ培养液对照组仅１例发生宫颈不典型增生（ＣＩＮⅠ），癌发率为零（０／３１）。在ＨＣＭＶ加巴豆油组，宫颈不典型增生的发生率为２６．４％（１４／５３）。而癌发率则提高到５２．８％（２８／５３）。此结果提示，人巨细胞病毒ＡＤ１６９株具有在体内转化宫颈内皮细胞，从而诱发宫颈癌的能力，巴豆油能够促进ＨＣＭＶ的诱癌能力，因此也讨论了ＨＣＭＶ致癌机制。
To study the relationship between human cytomegalovirus(HCMV) and cervical carcinoma, the mouse model that cervical carcinoma induced by UV inactivated HCMV and corton oil was used. Cotton tam pons, satured with UV inactivated HCMV liquid and control HEL medium were inserted into the vaginas of virgin Qunming mice 3 times a week, for 8 weeks. Then treated with corton oil 2 times a week for 4 weeks in the same way. After 20 weeks of exposure, these mice were sacrificed and serial sections of their repro ductive tracts were examined. Cervical dysplasia was detected by histological examination in 27.8% and cervical carcinoma in 20.5% of mice exposed to cytomegalovirus. In control mice, in contrast, dysplasia developed in 3.2% and carcinoma in none. In the group treated with corton oil and CMV, dysplasia was found in 26.4% and cervical carcinoma in 52.8%. Using χ 2 test, it is indicated that the differences are statistically significant. These data indicated that strain AD169 of cytomegalovirus could transform cervical epithelial cell in vivo, and it was oncogenic for the mouse cervix, and corton oil could promote the development of cervical carcinoma. The possible mechanism of the oncogenic was discussed.
基金
国家自然科学基金
关键词
子宫肿瘤
宫颈癌
巨细胞病毒
巴豆油
cytomegalovirus
oncogenic virus
corton oil
cervix neoplasma/ET
mice.
