摘要
目的探索动物眼视网膜的冰冻切片方法,以期得到高质量的切片。方法采用LeicaCM1900冰冻切片机,制作猪视网膜冰冻切片50余例,鼠视网膜冰冻切片130余例,观察其组织结构。结果去除其他组织及液体的影响是视网膜切片的关键,以保持良好的组织形态。结论所有组织和细胞切片的一个重要环节就是尽可能地保存组织和细胞以再现其生活状态,因此切片前固定、脱水及其他处理起着非常关键的作用。
Objective To find a proper method of getting frozen sections of animal retina with high quality.Methods We get more than 50 pieces of pig retinas and 130 pieces of rat retinas by using Leica CM1900 freezing microtome.Results We could get a good retina frozen section if only we can get rid of any impact from other tissues and fluid.Conclusion In order to get a better tissue structure,it is important to do a better preparation before section,such as fixation and dehydration.
出处
《眼科新进展》
CAS
2007年第11期831-832,共2页
Recent Advances in Ophthalmology
