摘要
为研究急性白血病（ＡＬ）多药耐药相关蛋白（ＭＲＰ）基因表达与临床耐药的关系，应用半定量的逆转录多聚酶链反应，检测了６５例ＡＬ患者骨髓细胞和１５例正常人外周血单个核细胞中ＭＲＰ基因的表达。以ＭＲＰ／β２Ｍ评价ＭＲＰ的表达水平，将ＭＲＰ／β２Ｍ≥０．３表达为ＭＲＰ＋。结果发现复发难治组ＭＲＰ的平均表达水平及阳性率最高，与正常对照组、初治组、长期生存组的ＭＲＰ平均表达水平及阳性率均有显著性差异（Ｐ＜０．０５），而长期生存期与正常对照组之间则无统计学差异，初治组ＭＲＰ＋病例与ＭＲＰ－病例的首次完全缓率之间有显著性差异，未观察到ＭＲＰ表达水平与ＦＡＢ分型有关。
Objective: To investigate the relationship between the expression of the multidrug resistance associated protein (MRP) gene in acute leukemia (AL) and clinical drug ressistance. Methods: Semi quantitative reverse transcriptase polymerase chain reaction was used to examine the expression of MRP gene in 65 bone marrow aspirates of AL and 15 normal expressed as ratio of MRP/β 2M(β 2 Microglobulin) and the ratio of MRP/β 2≥0.3 was defined as MRP +. Results: The level of MRP mRNA and the percentage of MRP + were significantly higher in refractory replased group than in normal control group, newly diagnosed group and long time survival group (P<0.05). But there was no significant difference between long time survival group and normal control group. The first complete remission (CR) rate of MRP - patients (84%) was significantly higher than MRP + (25%) samples in newly diagnosed group. There was no correlation between MRP expression and leukemia type. Conclusions: High expression of MRP leads to drug resistance and its an unfavorable factor to prognosis.
出处
《白血病》
1997年第1期20-23,共4页
