摘要
应用特异性单克隆抗体作DOT－ELISA和竞争ELISA检测65例脑囊虫病患者血清和脑脊液中游离循环Ag（FCAg）和循环免疫复合物抗原（CICAg）。血清FCAg阳性率分别为81．3％和81．5％（0．20～87．0μg／ml），CICAg阳性率为95.4％和92．3％（0.19～120.0μg／ml），脑脊液测定FCAg阳性单为78．5％和86.2％（0．32～25.0μg／ml），CICAg阳性率为90.8％和93.8％（0.19～46.4μg／ml）。结果表明检测总循环抗原（包括游离部分和复合物部分）有利于提高脑囊虫病的诊断。
Free and immune complex circulating antigens(FCAg and CICAg) sera and cerebrospinal fluid(CSF) from 65 neurocysticercosis patients were detected by DOT-ELISA and competitve ELISA using monoclonal antibodies. The positive rates (level) of FCAg were 81.3% and 81.5% (0. 2～87.0μg/ml) in sera and 78.5% and 86.2% (0.32～25. 0μg/ml) in CSF respectively;The positive rates(level) of CICAg were 95.4 % and 92.3% (0.19～128.0μg/ml) in sera and 90. 8% and 93. 8% (0. 19～46.4μg/ml) in CSF respectively. These results suggest that detection of total circulation antigen(FCAg and CICAg) from sera and CSF could contribute to diagnosis of neurocysticercosis.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1997年第3期36-37,共2页
Chinese Journal of Zoonoses
关键词
脑囊虫病
循环抗原
单克隆抗体
血清
脑脊液
Neurocysticerocosis
Circulating antigen
DOT-ELISA
Competitive ELISA
