摘要
利用高效毛细管电泳一场强放大柱内堆积技术分离测定磺胺嘧啶（SD）和磺胺甲噁唑（SMZ）。采用未涂层熔融石英毛细管柱（60．2cm×75μm，有效长度50cm），以50mmol／LNaH：PO4（pH6．0）为运行缓冲液，分离电压27．5kV，柱温25℃，检测波长214nm进行测定。进样前压力进水3．42kPa×12s；电动进样-10kV×9s。SD和SMZ的线性范围分别是0．05～10．00mg／L（r=0．9999），0．025～5.00mg／L（r=0．9994），检出限分别为1．74、1．39μg/L；将此方法应用于实际样品测定，SD回收率为98％～103％，SMZ回收率为97％-103％。
A simple and sensitive capillary electrophoresis method with field-enhanced stacking concentration for the analysis of sulfadiazine（SD） and sulfamethoxazole（SMZ） was developed. The sepa- ration was achieved with a fused-silica capillary（60. 2 cm ×75 μm, effective length was 50 cm） and a running buffer containing 50 mmol/L NaH2PO4（pH 6. 0）. The UV detection wavelength was 214 nm. The applied voltage was 27.5 kV, and the cartridge temperature was 25 ℃. Water plug was introduced from the anode by 3.42 kPa × 12 s before injection. Sample was injected by electrokinetic injection - 10 kV ×9 s. The linear range of the calibration curve for SD was 0. 05 - 10.00 mg/L with a correlation coefficient of 0. 999 9, and that for SMZ was O. 025 -5. 00 mg/L with a correlation coefficient of O. 999 4. The detection limits for SD and SMZ were 1.74 μg/L and 1.39 μg/L, respectively. The proposed method has been used to determine the SD and SMZ in the pharmaceutical preparations with a recovery range of 98% - 103% （SD） and 97% - 103% （SMZ）.
出处
《分析测试学报》
CAS
CSCD
北大核心
2007年第6期784-788,共5页
Journal of Instrumental Analysis
基金
泰山医学院博士启动基金资助项目(2006742)
关键词
毛细管电泳
场强放大样品堆积
磺胺嘧啶
磺胺甲噁唑
capillary electrophoresis
field-enhanced sample stacking
sulfadiazine
sulfamethoxazole
