摘要
目的:建立一种简便实用的胶体金免疫层析检测新方法,用于食品中黄原胶的快速筛查。方法:用黄原胶抗原标准品免疫新西兰大白兔,制备得到高效价的抗黄原胶多克隆抗体;采用柠檬酸三钠还原法制备胶体金,并将其与抗黄原胶多克隆抗体标记制得金标抗体;在硝酸纤维素膜检测线上,预包被黄原胶标准抗原,质控线上包被羊抗兔IgG,采用竞争法,制成黄原胶胶体金免疫层析检测试纸条,并对试纸条的特异性、敏感性和稳定性进行了试验。结果:该试纸条检测样品的敏感性为3g/kg。结论:研制出黄原胶胶体金免疫层析试纸条及其检测方法,该方法敏感性高、特异性强、操作简便,5min可出检测结果,可用于现场大量样品的快速检测。
Objective： To develop a new rapid colloid-gold immunochromatographic assay for the screening of xanthan gum in foods.Methods： The immunochromatographic assay was based on competitive immunoassay.Standard xanthan gum was used to immunize New Zealand rabbits to obtain high titer polyclonal antibodies.Colloid-gold was prepared according to the sodium citrate method,and was then conjugated to polyclonal antibodies.Xanthan gum antigen was coated on nitrocellulose membrane and goat anti-rabbit IgG was coated on control line.Results： The developed colloid-gold immnunochromatographic assay revealed a detection sensitivity of 3 g/kg.Conclusion： A new colloid-gold immunochromatographic strip for the detection of xanthan gum has been successfully developed.This method is highly specific,sensitive,rapid（the detection procedure can be completed in 5 min） and suitable for in situ,rapid,large-scale detection.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第20期270-273,共4页
Food Science
基金
北京市科研院科技创新工程项目(PXM2011_178305_113116)
关键词
黄原胶
胶体金免疫层析
快速检测
xanthan gum
colloid-gold immunochromatography
rapid screening
