摘要
优化了发酵培养肝素黄杆菌生产肝素酶的半合成培养基配方,种子液种龄48h,接种量10%,装量90ml/(750ml摇瓶),25℃培养32h。采用该优化方案,肝素酶的发酵水平可达1768u/L,较合成发酵培养基提高52%。
Heparinase production by Flavobacterium heparinum in a semidefined medium was studied and improved. When strain was cultured in a 750ml flask containing 90ml medium on a rotary shaker at 240r/min, 25℃ for 32h, the production of heparinase reached to 1768u/L which was increased by 52 % compared with that in the defined medium.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2007年第11期764-767,共4页
Chinese Journal of Pharmaceuticals
关键词
肝素酶
肝素黄杆菌
半合成培养基
发酵
heparinase
Flavobacterium heparinum
semidefinded medium
fermentation
