摘要
从植物种子中研究筛选出高产植酸酶的黑曲霉N2 5 ,进行了最适液体培养基的筛选 ,研究了黑曲霉N2 5在玉米半合成液体培养基中所产植酸酶的最适酶促反应条件。结果表明 :在四种培养基中 ,玉米半合成液体培养基为最适培养基 ,黑曲霉N2 5产植酸酶高峰期在 96h。黑曲霉N2 5所产植酸酶的酶促反应最适pH为 2 6和 4 6 ,并具有很好的热稳定性 ,一定浓度的Ca2 + 、Mg2 + 、Mn2 + 、Cr3+ 、Li+ 、EDTA和高磷是植酸酶活性的抑制剂 ,1 0nmol ml聚乙二醇 10 0 0 0、0 3nmol mLFe2 + 和低磷对植酸酶活性具有激活作用。
In this study, Aspergillus niger N25 ( A niger N25) highly-secreting phytase was isolated from soiland botanic seeds in P R China It was cultivated in corn,rice-meal and bran semi-synthesized culture mediums and one synthesized culture mediums producing phytase activity were 515 9nmol/ml·min,420 9nmol/ml·min,450 9 nmol/ml·min and 320nmol/ml·min,respectively,which indicated corn semi-synthesized culture medium was the best medium for A niger N25 When A niger N25 was cultivated in corn semi-synthesized culture medium for different time,phytase activity reached the highest level at 96h The optimum pH of A niger N25 phytase is 2 6 and 4 6 This enzyme had a broad optimum temperature range from 30℃ to 96℃,and showed a good heat stability Additionally,the certain concentration of Ca 2+ ,Mg 2+ ,Mn 2+ ,Cr 2+ ,Li +,EDTA and high concentration of phosphate inhibited the enzyme activity,while 0 3 nmol/ml Fe 2+ ,1 0mmol/ml polyethylene glycol 10000 and low concertration of phosphate can improve A niger N25 phytase activity
出处
《生物技术》
CAS
CSCD
2003年第3期20-22,共3页
Biotechnology
基金
国家"九五"科技攻关项目资助 (99- 0 0 9- 0 2 - 0 2 )
关键词
黑曲霉
植酸酶
酶促反应条件
维生素B
Aspergillus niger
phytase
reaction condition
phytase activity
