摘要
在红富士苹果（Ｍａｌｕｓｐｕｍｉｌａ）茎尖试管培养过程中，改变培养基中激素种类和浓度，进行光或暗处理，可诱导茎尖形成具有不同发育状态的组织和器官。对组织和器官进一步诱导，并进行内源ＩＡＡ和ＡＢＡ含量及比值、过氧化物酶同功酶、酯酶同功酶、可溶性蛋白带谱分析，结果表明：培养基中激素类型、浓度和组合决定了组织、器官的发育和分化方向；获得的各种发育状态的无根试管苗，其内源ＩＡＡ含量均极低，ＡＢＡ水平较为稳定，ＩＡＡ／ＡＢＡ约为１．０４～３．６３；有根试管苗中内源ＡＢＡ水平较高；分化根器官的愈伤组织ＩＡＡ水平高，ＡＢＡ水平较低，其比值为５．６～２１．９，而分化芽的愈伤组织中ＩＡＡ／ＡＢＡ比值小于１；ＩＡＡ和ＡＢＡ水平过低的愈伤组织继代时会逐渐褐化、死亡；暗处理可提高内源生长素水平；内源ＩＡＡ和ＡＢＡ、可溶性蛋白和酯酶同功酶之间在组织和器官分化诱导中呈现出一定规律性的联系。
The shoot tips of Fuji apple( Malus pumila )could develop into different tissues or organs when cultured on the media containing different plant growth regulators and treated with dark or light condition. The tissues or organs were continually induced to differentiate,and meanwhile some physiological indexes related to differentiation were measured. The indexes were endogenous IAA and ABA,peroxidase isozymes,esterase isozymes and soluable proteins. Results were that certain type,concentration and combination of plant growth regulators played a leading role for the differentiation. There were very low levels of endogenous IAA and stable levels of ABA in all shoots obtained after differentiation induction,and the ratio of IAA and ABA was about 1.04～3.63;After rooting,the shoots showed high level of ABA. The calli to differentiate into roots showed higher IAA and lower ABA level,and the ratio of IAA and ABA was about 5.6～21.9. On the contrary,the ratio of IAA and ABA was less than 1 in the calli to differentiate into buds;Calli containing very low IAA or ABA would become brown and die in their subculturing. Compared with light,dark condition could increase endogenous IAA of the cultures. There was a close relationship among exogenous 6-BA and NAA,endogenous IAA and ABA,esterase isozymes and soluble proteins in differention induction of the tissus and organs.
出处
《园艺学报》
CAS
CSCD
北大核心
1997年第3期229-234,共6页
Acta Horticulturae Sinica
关键词
红富士苹果
试管苗
生理指标
苹果树
器官分化
Fuji Apple
Endogenous IAA and ABA
Esterase isozymes
Soluble protein
Organ differentiation in vitro
