摘要
目的:观察阳和平喘颗粒对实验性支气管哮喘时炎症及组织的影响,探讨其治疗哮喘的机理。方法:采用卵蛋白成功诱发豚鼠哮喘后用阳和平喘颗粒进行治疗,ELISA法检测豚鼠血浆中可溶性白介素-2受体(sIL-2R)和白介素-6(IL-6)含量,放免法测定血浆中ET含量,并采用光镜和电镜法观察其对肺组织的影响。结果:模型组血浆中sIL-2R、IL-6和ET的含量与正常对照组比较明显增高(P<0.01),肺组织光镜和电镜下观察显示:模型组动物的肺组织可见嗜酸性粒细胞片状浸润,II型细胞部分线粒体脊突消失、膨胀,板层小体减少,有较多排空现象。肺组织光镜观察显示:血管腔狭窄,毛细血管内皮肿胀,核浓缩,胞浆内可见到髓样小体(细胞器变性),基底膜结构不清。阳和平喘颗粒高剂量可明显降低上述炎症介质,并可抑制肺组织中的炎症细胞浸润。结论:阳和平喘颗粒可能通过抗炎而达到抗实验性哮喘作用。
Objective： To observe the mechanism of effect of Yanghe Pingchuan granules on inflammation and the morphologic changes of tissue in guinespig experimental asthma. Methods ： The animal of asthma model is induced by OVA and treated by different dose of Yanghe Pingchuan granules. The concentration of IL - 6, slL - 2R and ET in serum are measured respectively by ELISA and by radioimmunity technique, and the effect of Yanghe Pingchuan granules on lung tissues are observed respectively by electron microscope and microscope. Results： The concentrations of slL - 2R, IL -6 and ET in serum in asthma group were higher than that in control group （ P 〈0.01 ）, Manifestation of the lung tis- sue under electron microscope ： the lung organism of the animal was infiltrated lamellarly by eosinophile granulocyte in the model set. A part of type Ⅱ cell′s mitochondrial crista disapeared, expanded, the lamellar body was reduced,with some empty cell. The lacuna vasorumis is becoming narrow, the capillary endothelium swell, the nucleus is concentrated, the elder pith corpuscle could be seen in cytoplasm （ denaturationof the organelle）, and the structure of basilar membrane isn t clear. Conclusion ： Yanghe Pingchuan granules may be can control asthma by anti - inflammation in asthmatic guinae pigs.
出处
《中华中医药学刊》
CAS
2007年第11期2246-2249,共4页
Chinese Archives of Traditional Chinese Medicine
关键词
实验性哮喘模型
阳和平喘颗粒剂
炎症
experimental asthma
Yanghe Pingchuan granules
inflammation
