摘要
采用反相高效液相色谱（RP-HPLC）法，Kromasil ODS-1柱，乙腈．水为流动相，流速1．0mL／min，柱温25℃，检测波长260nm，建立了肤痒胶囊中腺苷的含量测定方法．结果显示，腺苷的检测线性范围是0．021-0．525mg／mL，回归方程为A=331．9×10^5 P＋2．78×10^5，相关系数r为0．9995，回收率为99．55％，相对标准偏差为0．64％．因此，用HPLC法测定腺苷的含量，简便、快速、准确、可靠，可用于肤痒胶囊的质量控制．
A reversed-phase high-performance liquid chromatograghic method with photodiode array detection was developed for the contents determination of adenosine, Kromasil ODS-1 column was adopted, aeetonitrilewater was the mobile phase, flow velocity was 1.0 mL · min^- 1, column temperature was at 25 ℃ and detection wavelength was set at 260 nm for adenosine, to establish a contents determination method for Fuyang capsule. The results showed that the calibration curve had a good linearity within the range 0. 021 -0.525 mg · mL^-1 for adenosine, the regression equation was A = 331.9 × 10^5 p ＋ 2.78 × 10^5 with a coefficient 0. 999 5, and the average recovery was 99.55% with RSD of 0.64%. Therefore, the method was showed to be convenient, rapid, accurate, reliable to operate and suitable for the quality control of Fuyang capsule.
出处
《浙江师范大学学报（自然科学版）》
CAS
2007年第4期432-434,共3页
Journal of Zhejiang Normal University:Natural Sciences
