摘要
采用蛋白酶水解鲜鸡蛋蛋黄提取蛋黄油,通过响应曲面分析法建立蛋黄油提取率的二次多项数学模型,并考察酶添加比例、底物质量浓度和反应时间对蛋黄油提取率的影响,优化出蛋黄油提取率达到56.81%的工艺参数:酶添加比例为2.04%,底物质量浓度为20.28%,反应时间为14 h。
The procedure of extracting egg yolk oil from fresh egg yolk by protease was studied. Response surface methodology （RSM） was employed and a second order quadratic equation for extraction of egg yolk oil was built.The effects of percentage of pretease （Wt%, protease vs substrate）, concentration of substrate and time of reaction on extraction of egg yolk oil were explored. By analyzing the response surface plots and their corresponding contour plots as well as solving the quadratic equation, the optimum process parameters for extracting egg yolk oil were obtained as follows： the extraction rate of egg yolk oil is 56.81% under the condition of 2.04% of percentage of pretease, 20.28% of concentration of substrate, 14 h of time of reaction.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2007年第5期821-826,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
江西省教育厅科技计划项目(赣教技字[2006]244)
江西省农业厅农牧渔业科研计划项目(赣农字[2006]23)
关键词
蛋白酶
提取
蛋黄油
响应面分析法
pretease
extraction
egg yolk oil
response surface methodology （RSM）
