摘要
对刺齿贯众孢子萌发、原叶体增殖、孢子体的诱导进行了试验、观察和分析,研究了培养基和植物激素对其生长的影响。结果显示:成熟孢子在1/2 MS蔗糖浓度为1%培养基上59 d萌发90%以上,且有利于原叶体的形成。原叶体在1/2 MS+Kt0.5 mg/L培养基上60 d增殖速率可达1∶11。试管苗在1/2 MS+IBA2.0 mg/L的培养基上长势较好,根系粗壮且发达。该物种组培和快繁的成功,为它离体保存和持续利用提供了技术支撑。
The spore germination, prothallium proliferation and sporophyte inducement of Cyrtomium caryotideum were conducted, observed and analyzed. The impact of medium and plant hormones upon its growth had been studied. The results showed that over 90% of mature spores could germinate in 1/2 MS medium with 1% sucrose for 59 d. Such medium could improve the formation of prothalli. The proliferation ratio could reach 1：11 when prothalli were transferred in 1/2 MS ＋ Kt 0.5 mg/L medium for 60 d. The seedlings could grow well in 1/2 MS ＋ IBA 2. 0 mg/L medium, and develop brawny roots. The success of tissue culture and rapid propagation provided technical supporting for the in vitro preservation and sustainable uses of Cyrtomium caryotideum.
出处
《云南农业大学学报》
CAS
CSCD
2007年第6期795-798,共4页
Journal of Yunnan Agricultural University
基金
科技部国家科技基础条件平台项目(2004DKA30430
2005DKA21006)
关键词
刺齿贯众
组织培养
孢子体诱导
Cyrtomium caryotideum
tissue culture
sporophyte inducement
