摘要
采用热压方法制备了环烯烃聚合物（COP）微流控芯片。考虑到温度对微结构热压成形的质量影响最大,基于材料的粘弹性特性,通过变温准蠕变实验获得了热压参考温度Tr。实验证明,在该温度下热压成形,宽度和深度方向的复制精度分别达到了97.6%和94.3%。为了研究制备的COP微流控芯片的性能,将其和同一模具制备的PMMA微流控芯片进行了性能对比实验。通过背景荧光实验、电泳实验和DNA分析实验三方面的研究表明,与PMMA芯片相比,COP芯片背景荧光低,电泳效率高,检测重现性相对标准偏差小于2.5%,适用于生化分析。
Using hot embossing method, a cyclo-olefin polymer （COP） microfluidic chip was fabricated. The embossing reference temperature （Tr） was determined by the viscoelastic property of the COP, according to the variable temperature quasi-creep experiments. Experiments showed that hot embossing fulfilled at Tr temperature had high duplication accuracy and low substrate deformation, with average duplication accuracy of 97.6% in width and 94.3% in depth. Examinations concerning background fluorescence, electrophoresis and DNA separation and detection were carried, out on COP and PMMA （Polymethylmethacrylate） chip, respectively. The results showed that, compared with PMMA chip, COP chip has lower background fluorescence, higher electrophoresis efficiency and the relative standard deviations of reproducibility is less than 2.5 %. It indicates the fabricated COP microfluidic chip has potential to be used in biochemical analysis.
出处
《高技术通讯》
CAS
CSCD
北大核心
2007年第10期1050-1055,共6页
Chinese High Technology Letters
基金
国家863MEMS重大专项（2004AA404260）和辽宁省博士启动基金（20041073）资助项目.
关键词
COP微流控芯片
变温准蠕变实验
背景荧光
电泳效率
COP microfluidic chip, variable temperature quasi-creep experiment, background fluorescence, electrophretic efficiency
