摘要
目的对硅灰石涂层(CaSiO3)和二氧化钛涂层(TiO2)的生物学性能进行评价,为临床选择种植体表面改性方法提供实验依据。方法在纯钛表面采用等离子喷涂的方法分别加涂羟基磷灰石(HA)涂层、CaSiO3涂层和TiO2涂层,制作成1 cm×1 cm×0.2 cm的正方形块状物各78块,HA涂层组为阳性对照组,不加涂层的纯钛组(Ti)作为阴性对照组。通过兔骨髓基质干细胞诱导的成骨细胞体外培养的方法,在生物材料表面接种成骨细胞,用碱性磷酸酶染色和I型胶原染色对诱导的成骨细胞进行鉴定,测定细胞增殖曲线、细胞层碱性磷酸酶活性值和细胞总蛋白质含量,用倒置相差显微镜和扫描电子显微镜(SEM)进行细胞形态学观察。结果成骨细胞在二种新型涂层材料表面均能很好地增殖和分化,CaSiO3涂层和HA涂层的生物活性大于TiO2涂层(P<0.05)。结论Ca-SiO3涂层具有良好的生物活性,是一种较有应用前途的种植体涂层材料。TiO2涂层也有较好的生物相容性,但是其生物活性还有待于进一步提高。
Objective To evaluate the bioactivity and biocompatibility of two new implant coatings： the wollastonite coating （CaSiO3）and the titanium dioxide coating （TiO2） and to provide an experimental basis for clinical choice on type transformation methods of implant surfaces. Methods Plasma spraying to the implant surface with hydroxyapatite（HA） coating , wollastonite coating and titanium dioxide coating were used in experiments. The pure titanium without coating is used for negative comparison. The osteoblasts in vitro cultivation method was used to measure the growth curve, the alkalinity phosphate activeness of cellular layer, and the protein content of the bone cells; and scanning electron microscope （SEM） was used to observe the cytomorphology of cells, which might reflect the characteristics of the growth and the function of bone cells. Results The osteoblasts could grow well on the surface of all new coating materials. The order of their bioactivity was as follows： wollastonite coating, hydroxyapatite coating and titanium dioxide coating （ p 〈 0.05 ）. Conclusion The results show that wollastonite coating possesses good cytocompatibility and bioactivity, it is a quite promising implant coating material. The titanium dioxide coating also has the good biocompatibility, but its＇ bioactivity needs to be improved.
出处
《同济大学学报（医学版）》
CAS
2007年第5期30-34,39,共6页
Journal of Tongji University(Medical Science)
基金
上海市科委攻关项目(054119537)
关键词
种植体
涂层材料
生物学性能
成骨细胞培养
表面改性
implant
coating material
biology characteristics
osteoblasts cultivation
surface type transformation
