摘要
目的探求高效、经济、方便的新生大鼠下颌骨成骨细胞的原代培养方法,为研究下颌骨成骨细胞的相关特性奠定基础。方法无菌条件下取新生24h大鼠的下颌骨,采用组织块贴壁法培养成骨细胞,通过细胞形态学观察、碱性磷酸酶染色(alkaline phosphatase,ALP)、茜素红染色对细胞进行鉴定,并与颅骨分离的成骨细胞进行对比。结果所分离培养的细胞通过倒置相差显微镜观察呈梭型,碱性磷酸酶染色阳性,能形成钙结节,与颅骨分离的成骨细胞有相似的形态和相同的功能。结论采用组织块贴壁法成功分离出大量纯化的新生大鼠下颌骨成骨细胞,为体外研究下颌骨成骨细胞的特性奠定了实验基础。
Objective To explore an efficient,economical and convenient method for isolating neonatal rat mandibular osteoblasts.Methods Under sterile conditions,the mandibles were taken from neonatal rats which were born within 24 hours.Tissue block adherent method was used.ALP staining and alizarin red staining were used for identification.The mandibular osteoblasts were compared to the osteoblasts of skull.Results The cultured cells exhibited the typical morphological characteristics of osteoblasts.ALP staining was positive and alizarin red staining showed the formation of mineralized nudules.These cells had the similar shape and function with the osteoblasts of skull.Conclusion Osteoblasts of neonatal rat mandible were successfully isolated.This laid the foundation for in vitro study of osteoblasts of mandible.
出处
《北京口腔医学》
CAS
2011年第6期318-321,共4页
Beijing Journal of Stomatology
基金
国家自然科学基金资助项目(30830108)
关键词
成骨细胞培养
组织块贴壁法
新生大鼠
Osteoblast culture
Tissue block adherent method
Neonatal rat
