摘要
目的:提高人肺腺癌裸鼠皮下移植瘤的成瘤率。方法:人肺腺癌SPC-A-1、GLC-82培养细胞或相应的瘤组织匀浆接种于裸鼠皮下之前,先用富含生长因子的小鼠肉瘤S180腹水上清液按比例稀释,然后在裸鼠背部或腋窝皮下接种。观察人肿瘤细胞或肿瘤组织在加入小鼠肉瘤S180腹水上清液后,对移植瘤生长速度及成瘤率的影响。结果:加入小鼠肉瘤S180腹水上清液后,人肺腺癌SPC-A-1和GLC-82成瘤率明显提高,而且移植后的人肿瘤生长速度较未加小鼠肉瘤S180腹水上清液的人肺腺癌细胞明显加快。结论:小鼠肉瘤S180腹水上清液能明显提高人肺腺癌SPC-A-1和GLC-82裸鼠皮下移植瘤的成瘤率,促进肿瘤生长。
AIM：To improve tumor formation rate of xenografted human lung cancer in nude mice.METHODS：Before xenograftion,human lung cancer SPC-A-1 or GLC-82 cells and their tissue homogenate were diluted with supernatant of mice sarcoma 180 ascites being rich in growth factors.The diluted SPC-A-1 or GLC-82 cell suspensions were injected subcutaneously at right back or armpit of nude mice.The effects of supernatant of mice sarcoma 180 ascites on the tumor growth rate and tumor formation rate were evaluated.RESULTS：Compared with control group,the tumor growth rate and tumor formation rate of human lung cancer SPC-A-1 or GLC-82 in nude mice were significantly improved with the addition of the supernatant of mice sarcoma 180 ascites.CONCLUSION：The supernatant of mice sarcoma 180 ascites can significantly improve the tumor growth rate and tumor formation rate of human lung cancer SPC-A-1 or GLC-82 in nude mice.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2007年第9期1052-1057,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
人肺癌
裸小鼠
肿瘤模型
抑制率
human lung cancer
nude mice
tumor model
inhibition rate
