摘要
用气相色谱和高效液相色谱分别对长角血蜱雌蜱盾窝腺中性信息素z6-二氯酚（2,6－DCP）含量和整体蜕皮激素20-羟基蜕皮酮（20－E）含量进行了测定．结果表明：雌蜱2，6-DCP生物合成开始于由若虫蜕出后5d，之后其含量增加并在盾窝腺中沉积，吸血前后达到高峰（11．12ng·只(-1)），吸血后3d开始下降，但吸血后3-5d内保持在一恒定水平，此期内2，6－DCP释放以吸引雄蜱，吸血后7d即交配后含量急剧下降，饱血后下降为零．雌蜱由若虫蜕出后3d20－E有一峰（9．58ng·只(-1)），此峰可能与启动2，6－DCP合成有关，交配前20－E无明显变化；雌蜱申若虫蜕出后当天分别注射20－E和保幼激素Ⅲ（JHⅢ），均能使2，6－DCP合成量增加.以上结果说明,20-E和JHⅢ调节性信息素2，6－DCP的生物合成，蜕皮激素可能与2，6－DCP分泌无关．
Quantitative variation of the sex pheromone, 2,6-DCP, in fovtal gland andedysteroid (20-E) in whole body of female Haamaphysalis longicornis were detected by GCand HPLC separately. The results suggeStal that 2,6-DCP biosynthesis occurred on the 5thday aftermoulting from nymph, then it increased and deposited in the foveal gland andreached a peak(11.12 ng .tick-1) on the day just before or after feding. On the 3rd day afterfeeding, it declined and maitained at a relatively stable level between 3rd and 5th day afterfeeding. During this period, 2,6 -DCP released and exed the male searching behaviour. Aftermating, 2,6-DCP decrease signficantly and could not detected on the engorgement day. Apeak of 20-E was detected on the 3rd day aftermoulting from nymph. It wight initiate thebiosynthesis of 2, 6-DCP. Injdsn of exogenous20-E and JH III on the lst dayaftermoulting from nymph could enhance the quantity of 2,6-DCP. The above results indi cated that 2,6-DCP biosynthesis was controlled by hormones.
出处
《北京师范大学学报（自然科学版）》
CAS
CSCD
北大核心
1997年第1期130-134,共5页
Journal of Beijing Normal University(Natural Science)
基金
国家自然科学基金
关键词
长角血蜱
性信息素
生物合成
激素调控
Haemaphysalis longicornis, sex pheromone, 2, 6 -DCP
biosynthesis
hormonal adulation
