摘要
用固态发酵的方法研究了表面活性剂Tween-80和鼠李糖脂对堆肥中常见的放线菌栗褐链霉菌（Streptomyces badius）产酶及底物中挥发性有机质降解的影响.结果表明：添加0.05%的Tween-80能使微生物淀粉酶、蛋白酶和半纤维素酶最高酶活分别提高46.5%、65.9%和70.5%;添加0.15%的Tween-80能使微生物淀粉酶、纤维素酶和半纤维素酶最高酶活分别提高10.6%、51.3%和75%.添加0.006%的鼠李糖脂能使菌体产蛋白酶和半纤维素酶酶活分别提高14.6%和37.6%;鼠李糖脂浓度为0.018%时对微生物产酶有显著抑制作用.表面活性剂通过影响菌体产酶最终显著地影响了有机质的降解,对照组和添加了0.05%的Tween-80、0.15%的Tween-80、0.006%的鼠李糖脂、0.018%的鼠李糖脂的4组样品中挥发性有机质降解率分别为5.66%、7.65%、6.91%、5.07%、4.62%,表明表面活性剂对微生物产酶及有机物降解有显著影响.
The effects of the surfactants of Tween-80 and rhamnolipid on the enzymes production by Streptomyces badius and biodegradation of organisms were studied in a solid substrate fermentation （SSF） process. The results indicated that Tween-80 with the mass fraction of 0.05 % significantly increased the production of amylase, protease, xylanase from Streptomyces badius by 46.5%, 65.9%, 70.5%, respectively; Tween-80 with the mass fraction of 0.15% could promote Streptomyces badius to produce amylase, cellulose, xylanase obviously and the activities of enzymes were increased by 10.6%, 51.3 %, 75 %, respectively. Rhamnolipid with the mass fraction of 0.006% could increased the production of protease and xylanase from Streptomyces badius by 14.6% and 37.6%, respectively; rhamnolipid with the mass fraction of 0.018% inhibited the production of enzymes from Streptomyces badius. The organic matter samples＇ biodegradations of the groups of control, 0.05% Tween-80, 0.15% Tween-80, 0.006% rhamnolipid and 0.018% rhamnolipid were 5.66% ,7.65%, 6.91%, 5.07% and 4.62%, respectively, indicating that surfactants could influence the production of enzymes and biodegradation of orgarrisms obviously.
出处
《高技术通讯》
CAS
CSCD
北大核心
2007年第9期962-967,共6页
Chinese High Technology Letters
基金
863计划（2004AA649370）、973计划（2005CB724203）资助项目.
