摘要
采用ISSR-PCR分子标记技术对大菊85个品种的遗传多样性进行了分析。从77条ISSR引物中筛选出22条引物分别对供试材料基因组DNA进行扩增,共获得160条清晰可辨谱带,其中多态性带148条,多态位点百分率为92.5%,多态性较高。POPGENE软件计算结果表明,85个大菊品种平均有效等位基因数为1.6013,平均Nei's基因多样性指数为0.3485,平均Shannon信息指数为0.5162;应用SPSS软件计算得到各品种间的Jaccard相似系数介于0.294～0.802之间。UPGMA法将85个品种分成6个类群,聚类结果与品种瓣型相关。
ISSR-PCR was used to detect the genetic diversity and relationship of 85 chrysanthemum cultivars. ISSR fingerprinting amplified by 22 ISSR primers revealed a total number of 160 unambiguous bands, of which 148 ones were polymorphic and the polymorphism frequency was 92.5%. As analyzed by POPGENE 1.31, the average value of effective number of alleles, Nei＇s gene diversity and Shannon＇s information index were 1. 6013,0. 3485 and 0. 5162, respectively. As analyzed by SPSS 11.0, the similarity coefficient between cultivars ranged from 0. 294 to 0. 802. These 85 chrysanthemum cultivars were divided into six groups by UPGMA（unweighted pair group method with arithmetic average）based on Jaccard coefficient. The genetic relationship of 85 cultivars was related to their petal types.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第5期1243-1248,共6页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30400308)
上海市农委重点攻关项目[农科攻字(2006)第4-3]
关键词
菊花
ISSR
遗传关系
Dendranthema×grandiflora
ISSR
Genetic relationship
