摘要
对白蚁伞在摇瓶和发酵罐中发酵产生白蚁伞多糖的动力学做了较为详细的研究。比较了几种不同培养基产生白蚁伞多糖的差异，筛选出在２８℃，２００ｒ／ｍｉｎ条件下，摇瓶产生白蚁伞多糖的最适培养基为黄豆粉葡萄糖培养基（以ｇ／Ｌ计）：黄豆粉５，葡萄糖３０，酵母浸膏粉２，ＫＨ２ＰＯ４１，ＭｇＳＯ４７Ｈ２Ｏ０．５，自来水配制，自然ｐＨ值（约５．５）。发酵１２０ｈ，达到最大的多糖产量０．５７３ｇ／Ｌ。在ＮｅｗＢｒｕｎｓｗｉｃｋＳｃｉｅｎｔｉｆｉｃ（ＮＢＳ）Ｂｉｏｆｌｏ－Ⅱ６．６Ｌ的发酵罐上设定２８℃，６００ｒ／ｍｉｎ，５～７Ｌ／ｍｉｎ的通气量发酵２７ｈ，达到最大的多糖产量０．５６５ｇ／Ｌ。发酵过程的ｐＨ和氨基氮变化与其它食用菌的不同。对苯酚－硫酸法测定糖含量的方法作了改进。
The kinetic processes concerning polysaccharide produced by Termitomyces albuminosus CD 2 on a rotatory shaker and in a 6.6 L NBS fermentor were studied. Productivities of the polysaccharide obtained from media containing different nutrition indicated that a 5 g/L bean glucose medium is the best one for the polysaccharide production on the rotary shaker set at 28℃, 200 r/min. The highest yields of crude polysaccharide were 0.573 g/L in the shaker incubated with T . albuminosus CD 2 for 120 h and 0.565 g/L in the fermentor under conditions of 28℃, 600 r/min and an air flow rate of 5￣7 L/min for 27 h. The pH and amino N curves were different from the results reported in the literature, indicating a different growth behaviours of T. albuminosus CD 2. The phenol sulfuric acid method developed in this study adjusted the linearity of standard curve and led to the increasing precision of measuring the polysaccharide content in fermentation fluids.
出处
《清华大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1997年第6期54-57,共4页
Journal of Tsinghua University(Science and Technology)
基金
国家自然科学基金
香港 Croucher 基金
关键词
白蚁伞
多糖
发酵
食用菌
Termitomyces albuminosus
polysaccharide
fermentation
production
