摘要
目的:研究兔骨髓间充质干细胞(marrow-derived mesenchymal stem cells,MSCs)与纳米晶胶原基骨修复材料(nano-hydroxyapatite/collagen,NHAC)体外复合培养的结合程度,以及构建组织工程骨的可行性。方法:分离兔MSCs体外培养、纯化,取第3代MSCs与NHAC体外复合培养,第5,10 d后扫描电镜(SEM)观察二者复合程度。结果:兔MSCs贴壁生长,增殖速度快,生长曲线符合Logistic生长曲线;兔MSCs与NHAC体外复合培养,在第5,10 d进行扫描电镜观察,发现10 d时粘附于NHAC上的MSCs细胞数(38.52±7.21)明显高于5 d时粘附的细胞数(21.28±4.70),P<0.01。结论:兔MSCs与NHAC体外复合培养结合程度较高,可以用来构建组织工程骨,为进一步的动物实验奠定基础。
Objective： To explore the feasibility of rabbit marrow-derived mesenchymal stem cells （MSCs） and nano-hydroxyapatite/collagen （ NHAC ） in constructing tissue-engineering bone. Methods： Rabbit MSCs were isolated and purified in vitro, and the rabbit MSCs of 3^rd-generation were seeded onto prepared NHAC. Their adhesion situation was analyzed by scanning electron microscope（SEM） after 5,10 days. Results： Rabbit primary MSCs and passage MSCs g-rowed adherently with great reproductive activity, and the auxodrome of serial subcultivation MSCs was in accord with Logistic auxodrome. Rabbit MSCs were combined with NHAC to construct tissue-engineering bone in vitro, and by the SEM we found that at 10th day the amount（38.52±7.21 ） of MSCs adhered with NHAC was more than it（21.28±4.70） at 5th day, P 〈 0.01. Conclusion ： The adhesion degree of the compound of MSCs and NHAC was high, both of them can be used to construct the tissue-engineering bone for the secondary animal experiment.
出处
《江苏大学学报（医学版）》
CAS
2007年第5期389-392,I0001,共5页
Journal of Jiangsu University：Medicine Edition
基金
江苏大学科技发展基金资助项目(JLY20050008)
关键词
骨髓间充质干细胞
纳米晶胶原基骨修复材料
组织工程骨
扫描电镜
bone marrow-derived mesenchymal stem cells
nano-hydroxyapatite/collagen
tissue-engineering bone
scanning electron microscope
