摘要
目的评价TNF-α,IL-1β和IL-6促进铜绿假单胞菌、金葡菌和鲍曼不动杆菌体外生长作用。方法将铜绿假单胞菌、金葡菌和鲍曼不动杆菌菌液分别加入含有10、50、100、500 pg.1、10 ng TNF-α、IL-1β、IL-6的RPMI 1640培养液,孵育2、4、6、8、16～18h计数;另将中和量单克隆抗体与细胞因子混合后,做细菌定量培养。结果IL-6对铜绿假单胞菌、IL-1β对金葡菌以及IL-1β对鲍曼不动杆菌在共作用6h时表现为浓度依赖的生长促进作用;特异性细胞因子中和抗体则可显著抑制细胞因子对细菌生长的促进作用。结论细胞因子对细菌的生长促进作用,为迁延性炎症中细菌增殖的机制提供新的依据;而细胞因子对细菌的生长促进作用可被中和抗体所抑制,将为更有效进行抗菌治疗提供有益参考。
Objective To conduct an in vitro study of the growth of Pseudomonas aeruginosa, Staphylococcus aureus and Acinetobacter spp. , and evaluate their response to various concentrations of tumor necrosis factor （TNF）-α, interleukin （IL）-1β, and IL-6. Methods To monitor the growth of bacteria incubated with the cytokines TNFα, IL-I1β and IL-6 that were added to RPMI 1640 medium in various concentrations （10, 50, 100, 500 pg, 1 and 10 ng） at 2, 4 to 6, 8 and 16-18 h. The bacterial concentration was estimated when the mixtures of cytokines and specific neutralizing monoclonal antibodies （MoAbs） were incubated. Results We found that all three bacterial species showed concentration-dependent growth enhancement when incubated with one or more tested cytokines. Blockade by specific neutralizing cytokine significantly inhibited cytokine-induced growth. When compared with control, the 6 h growth response was maximal with IL-1β for Staphylococcus aureus and Acinetobacter spp. , and with IL-6 for Pseudomonas aeruginosa. Conclusions In this study we provide additional evidence for a newly described mechanism for bacterial proliferation in the presence of exaggerated and protracted inflammation. The effect that cytokine-induced growth enhancement inhibited by specific neutralizing cytokine MoAbs may be useful for antimicrobial therapy.
出处
《中国感染与化疗杂志》
CAS
2007年第5期344-347,共4页
Chinese Journal of Infection and Chemotherapy
