摘要
了解肿瘤坏死因子α（ＴＮＦ-α）在人体疾病发生中的作用．方法：建立了ＴＮＦ-α的双抗体放射免疫分析法（ＲＩＡ）．结果：方法的批内及批间ＣＶ分别为５．７６％±１．７３％和６．０８％±１．９１％．灵敏度为５．４ｐｍｏｌ／Ｌ．临床应用结果显示，癌症、细菌感染及脑血管病病人的血清ＴＮＦ-α值显著高于正常人（Ｐ＜０．０１），败血症、脑缺氧症新生儿的血清ＴＮＦ-α值显著高于正常新生儿（Ｐ＜０．０１）．结论：测定血清ＴＮＦ-α值有助于临床疾病的诊断及治疗．
PURPOSE To gain insight into the role of TNF-α in human diseases. METHODS Radioimmunoassay of TNF-α was developed. Concentration of TNF-α was measured in the serum from healthy subjects and patients. RESULTS Intraand inter- assay CVs were 5.76% and 6.08% respectively. The detection limit of this assay was 5.4pmol/ L. There was a significant difference in the serum concentration of TNF-α between healthy subjects and patients with cancers,bacterial infections, cerebral vascular diseases, neonatal septicemia, neonatal hypoxia induced encephalopathy (P< 0.01). CONCLUSIONS Radioimmunoassay of TNF-α is useful for the study and diagnosis of many diseases.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
1995年第3期161-163,共3页
Chinese Journal of Nuclear Medicine
