摘要
采用ＰＣＲＤＮＡ序列分析方法，对来自河南林州市３６例贲门癌组织进行肿瘤抑制基因Ｐ５３第４到９外显子突变分析。结果：３６例贲门癌组织中３例发生Ｐ５３基因突变（８％，３／３６）；突变分别发生在第５，６和８外显子上。对３例已知有Ｐ５３基因突变的癌组织大体标本，分别在４个不同部位（１２点、６点、９点和３点钟处）进行取材，进一步进行Ｐ５３突变结果分析，发现３例中第１例４个位点均发生Ｐ５３基因突变，且突变部位相同，第２例只有３个位点出现一致性改变，第３例仅在一个位点发现Ｐ５３基因突变，而其他３个部位未检测出Ｐ５３基因突变。提示：尽管这一地区食管癌和贲门癌发病率均较高，但两者发病的分子学基础及机理可能存在不同的途径。同一肿瘤不同部位Ｐ５３基因突变情况不一致，贲门癌变可能还有其它癌基因或肿瘤抑制基因参与。
Tumor suppressor gene P53 mutation was determined on exon 4 to 9 with PCR SSCP DNA sequence method on 36 subjects with gastric cardia adenocarcinoma from Linzhou City,Henan Province.The results demonstrated that there were 3 cases of P53 gene mutation (8%,3/36),which occurred on exon 5,6 and 8.In 3 cases of known P53 gene mutation,4 specimens of different sites (at 12,6,9 and 3 o’clock points)from each case were collected to further analyze the P53 mutation.The results showed that,in the first case,the same p53 gene mutation pattern was observed at the four different sites;in the second case,consistant P53 gene alteration was observed at three different sites and in the third case,P53 gene mutation was observed only at one site of specimen,and at the other three sites, no P53 gene mutation was found.The present results indicated that although there was a high incidence for both esophageal and gastric cardia cancer in this area,there might be different molecular basis and mechanistic pathway for these two diseases.Different P53 gene mutation in the same cancer tissue indicated that there might be other oncogene or tumor suppressor gene involved in gastric cardia cancer.
出处
《河南医科大学学报》
1997年第1期22-23,共2页
Journal of Henan Medical University
基金
国家教委留学回国人员专项基金
国家教委优秀青年教师基金
中美合作课题
