摘要
本文报道中国人杀菌蛋白（BPI）氨基端基因CDNA的克隆及序列测定。采用逆转录PCR方法，分别从HL-60细胞和正常人外周血、HGVRNA阳性献血员外周血的淋巴细胞中克隆了人BPI氨基端基因BG60、BGNor和BGHGV。序列分析结果表明：BG60与文献报道一致：BGNor有3个核苷酸差异，推导有1个氨基酸变化；BGHGV有3个核苷酸差异，其中一处与BGNor相同，推导有2个氨基酸变化。由此推测中国人BPI氨基端基因可能具有特殊性。
The genes, named BG6o, BGnor and BGHGV, encoding human N - terminal ofbactericidal / Permeability increasing protein (BPI) were cloned by RP- PCR method usingmRNA templates isolated from HL - 6O cells and Peripheral blood lymphocytes of a normalPerson and a blood donor with hepatitis G virus(HGV) RNA. The results of DNA sequencingshowed that the BG60 had the same sequence as that reported, but both BGNor and BGHGV hadthree nucleotide substitutions, which resulted in one and two amino acid variatons respectively.The results suggested that N - terminal gene of BPI exists diversity.
出处
《微生物学通报》
CAS
CSCD
北大核心
1997年第6期350-353,共4页
Microbiology China
基金
国家自然科学基金
