摘要
目的探讨乙酰肝素酶和CD105在大肠癌中的表达以及它们之间的关系。方法应用原位杂交方法检测乙酰肝素酶mRNA在95例大肠癌组织中的定位及表达;并用免疫组化方法对全部标本进行CD105染色,记数肿瘤微血管密度(microvesseldensity,MVD);分析乙酰肝素酶mRNA表达与大肠癌浸润、转移和血管生成之间的关系。结果95例大肠癌组织中,乙酰肝素酶mRNA阳性表达49例(51.57%),MVD平均值为(72.1±20.6);阴性表达46例(48.42%),MVD平均值为(41.3±12.4),乙酰肝素酶阳性组MVD表达与阴性组相比有显著性差异(P<0.01)。有浆膜浸润和伴淋巴结转移的大肠癌组织中,乙酰肝素酶mRNA表达阳性率分别为61.42%、63.49%,高于无浆膜浸润(24.00%)和无淋巴结转移组(28.12%),有显著差异(P<0.01)。结论乙酰肝素酶可促进大肠癌的浸润、转移和血管生成,可作为反映大肠癌生物学行为的客观指标。
Objective To study the expression of heparanase mRNA and CD105 in colorectal carcinoma and their correlation. Methods We detected the expression of heparanase mRNA by using ISH in 95 cases of colorectal carcinoma. Microvessel density （MVD） was detected by immunohistochemistry. Heparanase mRNA expression was analysed for its relationship to invasion depth, metastasis and MVD. Results MVD was 72. 1 ± 20. 6 in 49 （ 51.57% ） samples with positive heparanase mRNA, and 41.3 ± 12. 4 in 46 （ 48. 42% ） samples with negative heparanase mRNA, the difference being significant （P 〈 0. 01 ） . The positive rate of heparanase mRNA expression in colorectal carcinoma with serosal infiltration and lymph node metastasis was 61.42% and 63. 49%, higher than that without serosal infiltration （24. 00% ） and lymph node metastasis （28. 12% ）, the difference be- ing significant （P 〈0. 01 ） . Conclusion Heparanase facilitates colorectal carcinoma infiltration, lymph node metastasis and microvessel formation. Heparanase expression is an important predictor of the biological behavior of hu- man colorectal carcinoma.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2007年第4期470-473,共4页
Chinese Journal of Histochemistry and Cytochemistry
基金
河南省教育厅自然科学研究项目(20023100012)
关键词
乙酰肝素酶
大肠癌
血管生成
转移
Heparanase
Colorectal carcinoma
Angiogenesis
Metastasis
