摘要
为提高荇菜(Nymphoides peltatum)繁殖率,研究了荇菜的离体快繁技术。结果表明:带芽的嫩茎段为较适宜的外植体,升汞消毒时间以7min为宜;适宜的初代培养基为MS+BA0.5mg·L-1+NAA0.05mg·L-1;适宜的继代培养基为MS+BA1.0～1.5mg·L-1+NAA0.10～0.15mg·L-1;适宜的生根培养基为MS+NAA0.2mg·L-1。
The reproduction rate of Nymphoides peltatum is often very low when reproduced from seed or stem planting. In order to improve its reproduction rate, technology of in vitro propagation of Nymphoides peltatum was studied. The results indicated that the young stem segment with buds was the best explant; the optimum sterilization time with lg.L^-1 HgCl2 was 7 min; MS＋ BA0.5mg·L^-1＋NAA0.05mg·L^-1 was the optimum medium for primary culture; the most suitable medium for proliferation was MS＋ BA1.0-1.5mg·L^-1NAA0.10-0.15mg·L^-1 and MS＋NAA0.2mg· L^-1 was the best medium for root induction.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2007年第4期609-611,共3页
Journal of Shenyang Agricultural University
关键词
荇菜
离体繁殖
培养基
Nymphoides peltatum
in vitro propagation
medium
