摘要
目的:分析不同药用灵芝栽培菌株的遗传差异,为灵芝道地性研究、种质资源鉴定、亲缘关系研究、品种选育和引种栽培提供分子生物学依据。方法:以24个来源不同的灵芝及紫芝栽培菌株和野生菌株为材料,采用扩增酶切片断多态性AFLP方法,用NTSYSpc 2.1软件进行UPGMA法聚类分析,构建遗传系统树。结果:筛选14对引物,共扩增出177条多态性谱带。聚类结果表明利用AFLP技术可将全部供试材料区分开。结论:药用灵芝栽培菌株在分子水平上存在较大差异,并在相似系数0.676水平上聚为赤芝群和紫芝群。
Objective： To investigate the genetic diversity of Ganoderma cultivars provided for the genuineness study, germplasm resource identification, genetic relationship study, breeding, introduction and cultivante of Ganoderma strains. Method： With the software of NTSYSpc 2. 1, 24 materials, of G. lucidum and G. sinense, were studied using AFLP to construct the dendrogram. Resuit： There were 177 polymorphic bands with 14 primer combinations. And all materials could be identified with AFLP. Conclusion： There actually existed much genetic diversity at the molecular level among the germplasm resources of Ganoderma strains, and all the strains were clustered into G. lucidum group and G. sinense group at the similarity coefficient 0. 676.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第17期1733-1736,共4页
China Journal of Chinese Materia Medica
基金
四川省育种攻关项目(2001-11-06-18)
