摘要
目的：建立一种啤酒中赭曲霉毒素A的分析检测方法.方法：使用SPE-C18固相萃取,C18反相柱（250×4.6 mm）分离,乙腈-水-乙酸（99：99：2）为流动相,荧光检测器（激发波长333 nm,发射波长460 nm）检测.结果：啤酒不同水平的加标回收率为72.8%～87.0%,RSD均小于8.6%.并用该法测定了市售三种啤酒,均在检出限以下.赭曲霉毒素A标准溶液浓度0～200 ng/ml与峰面积呈良好线性关系,线性相关系数为0.9996.根据三倍噪声法.该法检出限为0.023 ng/ml.结论：使用SPE-C18柱极大地简化了样品预处理手续,可建立一种简便、快速、准确、实用的啤酒中赭曲霉毒素A的分析方法.
Objective：An analysis method detecting ochratoxin A in samples of beer was established. Methods：We adopted C18 cartridges to clean up the samples. The detection of OA was based on RP - HPLC with fluorescence detection（ HPLC - FL）. Mobile phase was acetonitrile - water - acetic acid （ 99 ： 99 ： 2 ）. The expiratory wavelength was 338 nm. The emission wavelength was 455 nm. Results：The samples＇ average rate of recovery was 72. 8% -87. 0%. The method had good ralativity （r =0. 9996） and good analytic precision （ RSD 〈 8. 6% ）. Utilizing 60 - fold concentration, the detection limit was 0. 023 ng/ml based on a signal - to - noise ratio of 3 ： 1. The method was evaluated by the recovery rate of OA in beers. The method was applied to analysis of 3 kinds of beer sold in Chin＆ The determination value was under the detection. Conclusion： Using SPE - C18 cartridges can shorten the sample pretreatment procedures and time significantly. This method is fast, simple, reliable and can be applied in the detection of ochratoxin A in beer.
出处
《中国卫生检验杂志》
CAS
2007年第8期1345-1346,共2页
Chinese Journal of Health Laboratory Technology
基金
河北省科技攻关项目(07276924)
