摘要
在pH值为0～1.5范围内,12-钨磷酸与盐酸苯海拉明(DP.HCl)反应形成缔合物后引起共振瑞利散射(RRS)强度显著增强,最大散射峰位于305nm,散射强度与DP.HCl浓度在8.0×10-9～1.6×10-6g/mL范围内成线性关系,据此建立了测定盐酸苯海拉明的RRS法。本法具有较高的灵敏度和良好的选择性,其检出限(3σ)为0.8×10-9g/mL。用于尿样中盐酸苯海拉明的测定,回收率为96.0%～104.2%。结合量子化学AM1法计算结果讨论了反应机理。
12-Tungstophosphoric acid reacted with diphenhydramine hydrochloride（DP · HCl） in an acidic medium pH 0 - 1.5 to form complexes, which resulted in great enhancement of resonance Rayleigh scattering （RRS）. The maximum RRS peak is at 305 nm, and the RRS intensity is proportional to the concentration of DP · HC1 in the range of 8.0 × 10^-9 - 1.6× 10^-6 g/mL. Therefore, a RRS method of determining DP · HCl was developed. This method has high sensitivity and good selectivity, and the detection limit（3σ） is 0. 8 × 10^-9 g/mL. The method was applied to determine the content of DP · HCl in urine samples, the recovery was between 96. 0% and 104. 2%. The reaction mechanism was discussed by means of AM1 of quantum chemistry.
出处
《应用化学》
CAS
CSCD
北大核心
2007年第9期1083-1085,共3页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(20475045)
重庆市科委资助项目
关键词
共振瑞利散射
钨磷酸
盐酸苯海拉明
resonance Reyleigh scattering,tungstophosphoric acid,diphenhydramine hydrochloride
